Analysis of Peptides & Proteins / LC & LCMS/MS

Application for LC & LCMS/MS

 
 
 

Simultaneous Analysis of Chiral Amino Acids - Produced by Intestinal Microbiota using LC/MS/MS

Some of the metabolites produced by intestinal microbiota are constantly absorbed from the intestinal lumen and transported throughout the entire body. In recent years, it has become clear that the intestinal microbiota contributes to the preservation and promotion of the hosts’ health and attention has been focused on the relationship of the intestinal microbiota and metabolites with health and diseases. For this reason, it is extremely important to analyze the metabolites produced by the intestinal microbiota. Amino acids have optical isomers (the D-isomer and L-isomer) and it has long been considered that only L-isomers are involved in the biological world. In this study, we comprehensively analyzed chiral amino acids in mouse colonic contents and blood plasma, using LC-MS/MS, and investigated D-amino acids produced by the intestinal microbiota.

 
 
 
 
 
 
 
 

A Single Injection LC-MS Analysis Scheme for Simultaneous Analysis of Biotherapeutics and Host-Cell Impurities via Online Digestion LC-MS/MS

Analysis of protein drug targets and impurities via LCMS involves time consuming offline protein digestion and separation. Automated protein digestion systems have addressed concerns of irreproducibility and improved laboratory efficiency by reducing human interaction with the sample. Although peptide mapping and Immuno-MS workflows are well established for on-line digestion, more complicated, orthogonal analyses have yet to be explored. Protein digestion of analyte proteins can be performed with or without affinity pulldown (depletion) of target proteins to enhance recovery of impurity proteins.

 
 
 
 
 
 
 
 

Peptide Mapping of Antibody Drugs by Nexera-i

Peptide mapping by HPLC is one of the important quality assurance tests used for verifying the primary structure of antibody drugs. Typically, following enzymatic digestion of the antibodies, separation is conducted using a traditional reversed phase column. Due to the large number of peaks that require separation, the use of small-particle columns and core shell columns for peptide analysis has spread in recent years. In order to compare elution profiles for identity and mutation confirmation, a highly repeatable system is required.

 
 
 
 
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