Methods & Technologies / Instrument Parameter & Validation

Applications for Instrument Parameters & Validation

Instrumental Validation of UV-Visible Spectrophotometer Compliant with JP, EP and USP

UV-Visible spectroscopy is adopted in the pharmacopoeia published in many countries. However, the differences of the instrumental validation items and their maximum tolerance required for a UV-visible spectrophotometer are seen among them. The newly developed UV-1900 UV-VIS spectrophotometer has the instrumental validation functions compliant with three major pharmacopoeias of Japanese Pharmacopoeia (JP), European Pharmacopoeia (EP) and United States Pharmacopeia (USP), and the instrumental validation based on them can be easily performed. The instrumental validation compliant with JP, EP and USP using the UV-1900 are described in this report.

 
 
 
 
 
 
  
 

Technical Report – Improved Linearity and Quantification Using the SPD-M40 Photodiode Array Detector

In principle, stray light generated during the UV-VIS and PDA detection process has a great influence on the linearity of the detector's response linearity. This report explains the influence of stray light upon detection and introduces the SPD-M40 photodiode array detector, which completely reduces the influence of stray light and achieves a linearity of 2.5 AU as a specification value (typical value is more than 2.5AU). Furthermore, in the low signal range, noise reduced noise has improved the detection accuracy of low concentrations, enabling the quantification of a wide concentration range. This enables simultaneous analysis and quantification of major components and impurities with different concentration ranges.

 
 
 
 
 
 
 
 
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