Automated Pretreatment Module for Cell Culture Media Analysis
Up to 65 samples can be pretreated automatically.
The probe for the culture supernatant sample ((3) sample probe) and the probe for the internal standard and organic solvent used as the deproteinization agent ((4) reagent probe) are separated, limiting cross contamination between samples.
Everything from pretreatment to analysis can be carried out with the common sample ID.
In the control software, the progress of pretreatment and analysis is easily confirmed.
Using the dedicated software, analysis operations are performed intuitively. No complicated method settings are required.
The Optimal Workflow Can Be Selected
The C2MAP system supports two modes: Batch mode (ordinarily used) that gives priority to the pretreatment of the culture supernatant samples placed, and sequential mode in which pretreatment and analysis are performed alternately for each sample.
Performing only the sample pretreatment process is possible in normal mode.
Supports a Wide Range of Measurement Compounds and Culture Supernatant Samples
The analysis method stored in the control software allows the simultaneous analysis of the following 95 components plus 2-Isopropylmalic acid.
It is confirmed that the C2MAP system can accommodate the follow ing culture media and culture media additives.
|Cell Type||CHO cells||iPS/ES cells||T cells||Mesenchymal Cell Type|
|Culture Media||BalanCD® CHO||AK03N||X-VIVO™ 10||MSCBM|
|1×CD CHO||Essential-8™||X-VIVO™ 15||MesenPRO™|
|Additives||Fetal Bovine Serum（100% v/v）|
For results obtained via LC/MS/MS, temporal changes in each component can be graphed by the dedicated viewer software. Analysts can monitor variations in metabolites secreted from cells and culture media components during cultivation, as well as display graphs of component comparisons with samples from different culture series. As a result, the consumption and depletion of culture media components, and changes in the amounts of metabolic components secreted from cells, can be observed, thereby providing useful insights into considerations of the optimal culture conditions and assessments of cellular status.
◆Temporal Changes in Measured Components
◆Measured-component Comparisons among Different Culture Series
For Research Use Only. Not for use in diagnostic procedures.
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